Creative Interdisciplinary Collaboration Program 2020
Assist. Prof. Takuya Mabuchi
|Development of high-sensitive method to detect aberrant proteins
Accumulation of peptide/protein aggregates is a common morphological feature, leading cause of many neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, and amyotrophic lateral sclerosis (ALS). A sensitive method for detecting protein aggregates in mixtures of blood and cell-derived components has not yet been established for these diseases. A development of an in situ peptide/protein aggregate detection method is robustly desired. The present research therefore focuses on the establishment of a new method for the sensitive detection of peptide/protein aggregation in cell-derived components by utilizing the amino acid residue-specific modification (e.g. cysteine and tyrosine) to understand the proteins aggregation mechanism in vivo.
This project is an interdisciplinary research that can be accomplished only by fusing different scientific disciplines and enhancing mutual understanding and cooperation among researchers from different fields; Dr. Mabuchi (Computational Science; Selection of compounds and molecules), Dr. Sato (Organic Chemistry; Synthesis of compounds), and Dr. Okumura (Protein Science; Biological evaluation). The significance of this interdisciplinary research proposal is to create novel molecules and develop strategies for the very early diagnosis of related diseases. We aim to apply the results of "detecting the aggregation process and the aggregation state of proteins/peptides" not only to basic researches in medicine, agriculture, biology, computational science, chemistry, and pharmacology, but also to detection methods and drug discoveries for definitive treatment of diseases related to proteins/peptides aggregations.