Research Projects
Creative Interdisciplinary Collaboration Program (Collaboration with Alumni) 2025
Asst. Prof. KANEMURA Shingo
| Title | Integrated Understanding of Protein–Protein Interactions through Dynamic and Quantitative Analysis |
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| Priod | 2025-2026 |
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This study aims to achieve a dynamic and quantitative understanding of the molecular mechanisms underlying protein–protein interactions. Specific associations between proteins, both intracellular and extracellular, serve as the foundation of essential biological processes such as signal transduction, metabolism, and immune responses. Elucidating these mechanisms at the molecular level is of critical importance for drug discovery, disease research, and molecular design. To date, our understanding of protein–protein interactions has primarily relied on static structural information obtained through methods such as X-ray crystallography. However, proteins are inherently dynamic entities, and it is increasingly recognized that their dynamic behavior plays a central role in determining function. I will employ biochemical and structural biology techniques to experimentally investigate protein–protein interactions. In parallel, Dr. Mabuchi (alumnus), as a co-researcher, will utilize computational approaches to identify interaction sites, characterize interfacial dynamics, track time-dependent changes in binding energy, and assess the influence of solvent environments—factors that are difficult to capture experimentally. By integrating these complementary perspectives, we will establish a high-precision, high-resolution analytical strategy that bridges experiment and theory. This research is expected to advance beyond the conventional paradigm based solely on static structures, enabling a comprehensive, dynamic, and functional understanding of protein–protein interactions and establishing general principles for their regulation. The outcomes will directly contribute to the development of innovative drug discovery strategies targeting protein interactions and the advancement of rational molecular design. Furthermore, this work will have a significant impact on both the academic development and practical applications of structural biochemistry and computational science. |
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