研究業績 Publication list
Shinsuke Niwa's Publication list
原著論文と総説 (Original Papers and Reviews)
2023年
43.Chiba Kyoko & Niwa Shinsuke (2023)
Autoinhibition and activation of kinesin-1 and their involvement in amyotrophic lateral sclerosis
Current Opinion in Cell Biology in press
42.
Kita Tomoki, Sasaki Kazuo,
Niwa Shinsuke (2023)
Modeling the motion of disease-associated KIF1A heterodimers
Biophysical Journal 122 (22):356-366.
https://www.cell.com/biophysj/fulltext/S0006-3495(23)00650-1
41.Kita Tomoki, Chiba Kyoko, Wang Jiye, Nakagawa Atsushi, Niwa Shinsuke (2023)
Comparative analysis of two Caenorhabditis elegans kinesins KLP-6 and UNC-104 reveals common and distinct activation mechanisms in kinesin-3
elife 12:RP89040
40.Niwa Shinsuke & Chiba Kyoko (2023)
Generation of recombinant and chickenized scFv versions of an anti-kinesin monoclonal antibody H2
Cytoskeleton 80 :356-366.
- Article selected as Cover
39.Chiba Kyoko, Kita Tomoki, Anazawa Yuzu, Niwa Shinsuke (2023)
Insight into the regulation of axonal transport from the study of KIF1A-associated neurological disorder (KAND)
Journal of Cell Science 136 (5):jcs260742.
DOI:https://doi.org/10.1242/jcs.260742
38.Higashida Maki & Niwa Shinsuke (2023)
Dynein intermediate chains DYCI-1 and WDR-60 have specific functions in Caenorhabditis elegans
Genes to Cells 28(2):97-110.
DOI:https://doi.org/10.1111/gtc.12996
2022年
37.Taguchi Shinya#(# equal contribution), Nakano Juri#(# equal contribution), Imasaki Tsuyoshi#(# equal contribution), Kita Tomoki, Saijo-Hamano Yumiko, Sakai Naoki, Shigematsu Hideki, Okuma Hiromichi, Shimizu Takahiro, Nitta Eriko, Kikkawa Satoshi, Mizobuchi Satoshi, Niwa Shinsuke*, Nitta Ryo* (2022)
Structural model of microtubule dynamics inhibition by kinesin-4 from the crystal structure of KLP-12 -tubulin complex
eLife 11 e77877 (* corresponding authors)
https://elifesciences.org/articles/77877
36.Anazawa Yuzu#, Kita Tomoki# (# equal contribution), Iguchi Rei, Hayashi Kumiko and Niwa Shinsuke(2022)
De novo mutations in KIF1A associated neuronal disorder (KAND) dominant negatively inhibit axonal transport of synaptic vesicle precursors.
Proceedings of the National Academy of Sciences, USA 119 (32) e2113795119
https://doi.org/10.1073/pnas.2113795119
35.Imasaki Tsuyoshi#, Kikkawa Satoshi#(# equal contribution), Niwa Shinsuke*, Saijo-Hamano Yumiko, Shigematsu Hideki, Aoyama Kazuhiro,Mitsuoka Kaoru,Shimizu Takahiro, Aoki Mari, Sakamoto Ayako, Tomabechi Yuri,Sakai Naoki, Shirouzu Mikako, Taguchi Shinya, Yamagishi Yosuke, Setsu Tomiyoshi, Sakihama Yoshiaki, Nitta Eriko, Takeichi Masatoshi, Nitta Ryo* (2022)
CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation
eLife 11:e77365 (* corresponding authors)
https://elifesciences.org/articles/77365
34. Nakano Juri#, Chiba Kyoko#(# equal contribution)* and Niwa Shinsuke*(2022) An ALS-associated KIF5A forms oligomers and aggregates and induces toxicity in neurons
Genes to Cells 27(6):421-435.
DOI: 10.1111/gtc.12936 (* corresponding authors)
33. Chiba Kyoko, Ori-McKenney M. Kassandara, Niwa Shinsuke, McKenney J. Richard(2022) Synergistic autoinhibition and activation mechanisms control kinesin-1 motor activity
Cell Reports 39(9):110900
DOI: doi: 10.1016/j.celrep.2022.110900
32. Anazawa Yuzu and Niwa Shinsuke (2022) Analyzing the impact of gene mutations on axonal transport in Caenorhabditis elegans.
Methods in Molecular Biology 2431:465-479
2021年
31. Lam J. Aileen, Rao Lu, Anazawa Yuzu, Okada Kyoko, Chiba kyoko, Davis Mariah, Niwa Shinsuke, Gennerich Arne, Nowakowski W. Dan, McKenney J. Richard (2021) A Highly Conserved 310-Helix Within the Kinesin Motor Domain is Critical for Kinesin Function and Human Health.
Science Advances 7(18)
DOI: 10.1126/sciadv.abf1002
30. Hayashi Kumiko, Miyamoto G. Miki, Niwa Shinsuke (2021) Effects of dynein inhibitor on the number of motor proteins transporting synaptic cargos.
Biophysical Journal
DOI:https://doi.org/10.1016/j.bpj.2021.02.018
29. Takahashi Hironori, Kamiya Mako, Kawatani Minoru, Umezawa Keitaro, Ukita Yoshiaki, Niwa Shinsuke, Oda Toshiyuki Urano Yasuteru (2021) Neural and behavioral control in Caenorhabditis elegans by a yellow-light-activatable caged compound
Proceedings of the National Academy of Sciences, USA 118 (6) e2009634118
DOI:https://doi.org/10.1073/pnas.2009634118
2020年
28. Hayashi Kumiko, Hasegawa Shin and Niwa Shinsuke (2020) What is the temperature of a cell?
Europhysics News 51/5 p.48 - p.50 (Review)
DOI:https://doi.org/10.1051/epn/2020510
27. Monroy Y. Brigette, Tan C. Tracy, Oclaman May, Han S. Jisoo, Simo Sergi, Niwa Shinsuke, Nowakowsky W. Dan, McKenney J. Richard, Ori-McKenney M. Kassandra (2020) A combinatorial MAP code dictates polarized microtubule transport.
Developmental Cell
DOI:https://doi.org/10.1016/j.devcel.2020.01.029
2019年
26. Gabrych R. Dominik, Lau Victor, Niwa Shinsuke, Silverman A. Michael (2019) Going Too Far Is The Same As Falling Short* Kinesin-3 family members in hereditary spastic paraplegia
*Confucius 551 BC- 479 BC
Frontiers in Cellular Neuroscience 13 p.419 (Review)
https://www.frontiersin.org/articles/10.3389/fncel.2019.00419/abstract
25. Chiba Kyoko, Takahashi Hironori, Chen Min, Obinata Hiroyuki, Arai Shogo, Hashimoto Koichi, Oda Toshiyuki, McKenney J. Richard*, Niwa Shinsuke* (2019) Disease-associated mutations hyperactivate KIF1A motility and anterograde axonal transport of synaptic vesicle precursors.
Proceedings of the National Academy of Sciences, USA
116(37) 18429-18434
(* corresponding authors)
https://www.pnas.org/content/early/2019/08/26/1905690116
24. Shao Zhenhua, Niwa Shinsuke , Higashitani Atsushi, Daigaku Yasukazu (2019) Vital roles of PCNA K165 modification during C. elegans gametogenesis and embryogenesis.
DNA Repair 82 doi.org/10.1016/j.dnarep.2019.102688
23. Yanagi Shota, Sugai Tomoya, Noguchi Takuma, Kawakami Masao, Sasaki Makoto, Niwa Shinsuke, Sugimoto Asako, Fuwa Haruhiko (2019) Fluorescence-labeled neopeltolide derivatives for subcellular localization imaging.
Organic & Biomolecular Chemistry doi: 10.1039/c9ob01276a.
22. Hayashi Kumiko, Matsumoto Shiori, Miyamoto G Miki, Niwa Shinsuke (2019) Physical parameters describing neuronal cargo transport by kinesin UNC-104.
Biophysical Reviews doi:10.1007/s12551-019-00548-9.(Review)
2018年
21. Xu Fang, Takahashi Hironori, Tanaka Yosuke, Niwa Shinsuke, Wicklund P. Matthew, Hirokawa Nobutaka (2018) An IGF1R-binding domain of KIF1Bβ affected in hereditary neuropathy serves for neuronal development.
Journal of Cell Biology 217(10): 3480-3496.
20. Obinata Hiroyuki, Sugimoto Asako, Niwa Shinsuke (2018) Streptothricin acetyl transferase 2 (Sat2): a dominant selection marker for Caenorhabditis elegans genome editing,
PLoS One, 13(5):e0197128. doi: 10.1371/journal.pone.0197128. eCollection 2018.
19. Chia Poh Hui#, Zhong L. Franklin#, Niwa Shinsuke#(#equal contirbution), Bonnard Carine, Utami H. Kagistia, Zeng Ruizhu, Lee Hane, Eskin Ascia, Nelson F. Stanley, Xie H. William, Al-Tawalbeh Salmah, El-Khateeb Mohammad, Shboul Mohammad, Pouladi A. Mahmoud, Al-Raqad Mohammed Reversade Bruno (2018) A homozygous loss-of-function CAMK2A mutation causes growth delay, frequent seizures and severe intellectual disability.
eLife, 7. pii: e32451. doi: 10.7554/eLife.32451.
18. Hayashi Kumiko, Hasegawa Shin, Sagawa Takashi, Tasaki Sohei, Niwa Shinsuke (2018)
Non-invasive force measurement reveals the number of active kinesins on a synaptic vesicle precursor in axonal transport regulated by ARL-8.
Phys Chem Chem Phys. 20(5), 3403-3410.
2017年
17. Niwa Shinsuke (2017) Immobilization of Caenorhabditis elegans to observe intracellular transport in neurons.
Journal of Visualized Experiments 18;(128). doi: 10.3791/56690.
16. Niwa Shinsuke#*, Tao Li#, Lu Y. Sharon#(#equal contirbution), Liew M. Gerald, Feng Wei, Nachury V. Maxence*, Shen Kang* (2017) BORC regulates the axonal transport of synaptic vesicle precursors by activating ARL-8.
Current Biology 27(17), 2569-2578,(* Corresponding authors)
15. Niwa Shinsuke, Nakamura Fumio, Tomabechi Yuri, Aoki Mari, Shigematsu Hideki, Hirokawa Nobutaka, Goshima Yoshio, Nitta Ryo (2017) Structural basis for CRMP2-induced axonal microtubule formation.
Scientific Reports 7, 10681, doi: 10.1038/s41598-017-11031-4
2016年
14. Niwa Shinsuke, Lipton M. David, Morikawa Manatsu, Charles Zhao, Hang Lu, Hirokawa Nobutaka and Shen Kang (2016) Autoinhibition of a neuronal kinesin UNC-104/KIF1A regulates the size and density of synapses.
Cell Reports 16, 2129-214
13. Niwa Shinsuke (2016) Nephronophthisis-related gene ift-139 is required for the ciliogenesis in Caenorhabditis elegans.
Scientific Reports 6, 31544; doi: 10.1038/srep31544
12. Tanaka Yosuke#, Niwa Shinsuke#, Dong Ming#, Farkhondeh Atena , Wang Li, Zhou Ruyun, and Nobutaka Hirokawa (2016) The molecular motor KIF1A transports the TrkA neurotrophin receptor and is essential for sensory neuron survival and function.
Neuron 90, 1215-29 (#These authors equally contributed to this work)
学際研着任前の論文、総説 (old papers and reviews)
11. Farkhondeh Athena, Niwa Shinsuke, Takei Yosuke, Hirokawa Nobutaka. (2015) Characterizing KIF16B in neurons reveals a novel intramolecular "stalk inhibition" mechanism that regulates its capacity to potentiate the selective somatodendritic localization of early endosomes.
Journal of Neuroscience 35, 5067-86
10. Niwa Shinsuke (2015) Kinesin superfamily proteins and the regulation of microtubule dynamics in morphogenesis.
Anatomical Science International 90, 1-6.
9. Niwa Shinsuke#, Takahashi Hironori# and Hirokawa Nobutaka. (2013) β-tubulin mutations that cause severe neuropathies disrupt axonal transport.
The EMBO Journal 32, 1352-1364. (#These two authors equally contributed to this work)
-Article highlighted in "News and Views" section in The EMBO Journal.
Gumy LF and Hoogenraad CC "Off the rails: axonal cargos on the road to nowhare" The EMBO Journal 32, 1345- 1347 (2013).
8. Zhou Ruyun#, Niwa Shinsuke#, Guillaud Laurent, Tong Ying and Hirokawa Nobutaka. (2013) A molecular motor, KIF13A, controls anxiety by transporting the serotonin type 1a receptor.
Cell Reports 3, 509-519 (#These two authors equally contributed to this work)
7. Niwa Shinsuke, Nakajima Kazuo, Miki Harukata, Minato Yusuke, Wang Doudou and Hirokawa Nobutaka. (2012) KIF19A is a microtubule depolymerizing kinesin for ciliary length control.
Developmental Cell 23, 1167-1175.
- Article selected as Cover
- Article highlighted in "Research Highlight" section in Nature Reviews Molecular and Cell Biology (Nature Reviews Molecular and Cell Biology 14, 4).
6. Noda Yasuko#, Niwa Shinsuke#, Homma Noriko, Fukuda Hiroyuki, Imajoh-Ohmi Shinobu, Hirokawa Nobutaka (2012) Phosphatidylinositol 4-phosphate 5-kinase alpha(PIPKα) regulates neuronal microtubule depolymerase kinesin, KIF2A and suppresses the elongation of axon branches.
Proceedings of the National Academy of Sciences 109, 1725-1730. (#These two authors equally contributed to this work)
5. Nakata Takao, Niwa Shinsuke, Okada Yasushi, Perez Franck, Hirokawa Nobutaka (2011) Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport.
The Journal of Cell Biology 194, 245-255.
4. Hirokawa Nobutaka, Niwa Shinsuke, Tanaka Yosuke (2010) Molecular Motors in Neurons: Transport Mechanisms and Roles in Brain Function, Development and Disease.
Neuron 68, 610-638. (Review)
3. Zhou Ruyun#, Niwa Shinsuke#, Homma Noriko, Takei Yosuke, Hirokawa Nobutaka (2009) KIF26A is an unconventional Kinesin and regulates GDNF-Ret signaling in enteric neuronal development.
Cell 139, 802-813. (#These two authors equally contributed to this work)
2. Hirokawa Nobutaka, Noda Yasuko, Tanaka Yosuke, Niwa Shinsuke (2009) Kinesin superfamily motor proteins and intracellular transport.
Nature Reviews Molecular Cell Biology 10, 682-696. (Review)
- Review Article selected as Cover
1. Niwa Shinsuke, Tanaka Yosuke, Hirokawa Nobutaka (2008) KIF1Bβ- and KIF1A-mediated axonal transport of presynaptic regulator Rab3 occurs in a GTP-dependent manner through DENN/MADD.
Nature Cell Biology 10, 1269-1279.
- Article highlighted in "Leading Edge" section in Cell (Cell 135, 373-375; 2008)
学会発表
招待講演 (Invited lectures and seminers)
13. Niwa Shinsuke "Activation of KIF1A/UNC-104 motor and its involvement in synaptogenesis and neurological disorders"
Cytoskeleton in Neuron and Glia Webinar, online, 2023年9月28日(EST)
12. Niwa Shinsuke "Increased and Decreased axonal transport of synaptic precursors is
associated with neurological disorders"
Tohoku Neurotech Symposium 2023、東北大学片平キャンパス、仙台、 2023年7月31日
11.
Niwa Shinsuke"Activation of KIF1A/UNC-104 motor and its involvement in neurological disorders"
第49回内藤コンファレンス「Frontiers of Microtubule and its-related motors」 シャトレーゼガトーキングダム札幌、札幌、 2023年7月5日
10. Niwa Shinsuke "De novo mutations in KIF1A associated neuronal disorder (KAND) dominant negatively inhibit axonal transport of synaptic vesicle precursors."
Kif1a.org meeting, online, 2022年3月25日
9. 丹羽伸介 "Analyses of KIF1A-associated neuronal disorder by genetics and single molecule assays"
第59回日本生物物理学会 シンポジウム オンライン 2021年11月25日
8. 丹羽伸介 "Disease-associated mutations in human KIF1A overactivate motility of KIF1A and anterograde axonal transport of synaptic vesicle precursors"
第42回日本神経科学会大会 シンポジウム 新潟国際会議場、新潟、 2019年7月25日
7.Niwa Shinsuke "Regulation of axonal transport and Diseases" Peking University（北京大学）, Beijing, China, Arp.23rd, 2019
6.Niwa Shinsuke "Regulation of axonal transport and Diseases" Tsinghua University(清華大学), Beijing, China Apr. 19th, 2019
5.Niwa Shinsuke "Regulation of axonal transport and Diseases" UC Davis seminer, University of California, Davis, CA, USA Jan. 20th, 2019
4.Niwa Shinsuke "The molecular mechanism of the axonal transport of synaptic vesicles and Diseases" Neuroscience Research Colloquia, Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada. Oct. 20th, 2017
3. 丹羽伸介「線虫遺伝学を用いた分子モーター研究」
第６９回日本細胞生物学会大会 シンポジウム 仙台国際会議場、仙台、２０１７年６月１５日
2. 丹羽伸介「線虫遺伝学を用いた軸索輸送研究」
第１２２回日本解剖学会大会 長崎大学医学部、長崎、２０１７年３月２８日
1. 丹羽伸介KIF19A regulates the length of cilia by deporimerizing microtubules
第６９回日本細胞生物学会大会シンポジウム ､ 2013年6月
その他
